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KMID : 0861020030180010079
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Korea Journal of Herbology
2003 Volume.18 No. 1 p.79 ~ p.85
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Physicochemical Study of Cervi Parvum Cornu Processed by Lyophilization
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Abstract
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The non-mature antlers of deer, Cervi Parvum Cornu (CPC), have been used as a tonic in traditional Korean medicine.
Objectives: The purpose of this study was to evaluate the quality of Cervi Parvum Cornu which is newly processed by lyophiliztion. We compared lyophilized CPC (LCPC) with parboiled CPC (PCPC) by the yield of water extract, the contents of total free amino acids and the patterns of gangliosides.
Methods: We analyzed the quantitiative and qualitative determinations of amino acids in LCPC using HPLC method. The contents and patterns of gangliosides in LCPC were measured by gravimetric analysis and HPTLC.
Results: The yields of the water extract from LCPC and PCPC gave 18% and 10%, respectively. The contents of total free amino acids in the PCPC were much higher than the LCPC. Comparing the TLC pattern of gangliosides, the both samples showed similar patterns as the purchased gangliosides. The contents of gangliosides in the LCPC (18.6 mg/g) were higher than those in the PCPC (14.3 mg/g).
Conclusions: These results suggest that the lyophilizing process may be an alternative method for improving the quality of CPC.
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